Your Very Own DNA Lab - Fun at Parties!


	Your Very Own DNA Lab - Fun at Parties!

knslyr

10-11-2007 10:33 AM

713 views

tags:

kuro5hin, dna, science, party tricks

knslyr says:

Wanna see some DNA? This is a fun little demo that works well at parties.

Must be some BUTT NAKED CRAZY ASS PARTIES!!!

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<div style="margin: 12px 0px; font-family: arial; color: #333333; background: #ffffff; border: solid 4px #e5e5e5; width: 100%; clear: left;"><div class="CM_CTB_Content_Wrap" style="margin: 0px; padding: 0px;background-color: #ffffff;"><div style="border-bottom: solid 1px #dcdcdc; white-space: nowrap; margin-bottom: 8px; background-color: #eeeeee ;background-image: url(http://clipmarks.com/images/source-bg.gif); background-repeat: repeat-x; height: 24px; line-height: 24px; vertical-align: middle; padding-bottom: 4px; color: #666666; font-size: 10px;" ><a href="http://clipmarks.com/clip-to-blog/" title="see clips that are hot right now"><img src="http://content.clipmarks.com/blog_embed/b7f27910-223e-41c2-b5ae-fda63cf36023/3734F4F2-7390-40AD-B4A3-A80E6FEA54DA/" alt="" width="19" height="19" border="0" style="vertical-align: middle; margin: 0px 4px; display: inline; border: none; float:none;" /></a>clipped from <a title="http://www.kuro5hin.org/story/2007/10/2/114918/141" href="http://www.kuro5hin.org/story/2007/10/2/114918/141" style="font-size: 11px;">www.kuro5hin.org</a></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.kuro5hin.org/story/2007/10/2/114918/141"><table background="undefined" bgcolor=""><tr><TD class="justified" colspan="2"><P>
			<FONT size="2" face="verdana, arial, helvetica, sans-serif">
YOU WILL NEED:
</FONT></P><UL><FONT size="2" face="verdana, arial, helvetica, sans-serif"><li style="margin-left:16px;padding-left: 0px;">A strawberry
    </LI><li style="margin-left:16px;padding-left: 0px;">Some powdered meat tenderizer (papain)
    </LI><li style="margin-left:16px;padding-left: 0px;">Some liquid soap
    </LI><li style="margin-left:16px;padding-left: 0px;">A fork
    </LI><li style="margin-left:16px;padding-left: 0px;">Two small cups (shot glasses are great)
    </LI><li style="margin-left:16px;padding-left: 0px;">A coffee filter
    </LI><li style="margin-left:16px;padding-left: 0px;">Some rubbing alcohol</LI></FONT></UL>
<FONT size="2" face="verdana, arial, helvetica, sans-serif">			</FONT>
			<P></p> <P>
			<FONT size="2" face="arial, Helvetica, Sans-Serif"> </FONT>
			</p> </TD></tr></table></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.kuro5hin.org/story/2007/10/2/114918/141"><FONT size="2" face="verdana, arial, helvetica, sans-serif">This extracts DNA from a strawberry. You can use just about any soft fruit, but strawberries are best; they're octoploid (each cell has 8 sets of chromosomes), so there's a shitload of DNA in them. Basically, you just squish up the strawberry in a little bit of water and add a touch<SUP>*</SUP> each of soap and meat tenderizer. The soap disrupts the membranes and the protein secondary & tertiary structure in the tissue, allowing access to the DNA. The meat tenderizer contains proteases (papain, typically) that chew up the proteins that bind to the DNA, helping it get out into the solution. It also chews up the DNAses in the prep. The coffee filter gets rid of the trash, cleaning up the solution. The isopropyl alcohol (rubbing alcohol) then precipitates the DNA out of solution. Here's the rundown:
</FONT></blockquote></div><div style="margin: 0px 6px 6px 4px;"><table style="font-size: 11px;border-spacing: 0px;padding: 0px;" cellpadding="0" cellspacing="0" width="100%"><tr><td style="background:transparent;border-width:0px;padding:0px;">&nbsp;</td><td align="right" style="background:transparent;border-width:0px;padding:0px;width:107px" width="107"><a href="http://clipmarks.com/share/3734F4F2-7390-40AD-B4A3-A80E6FEA54DA/blog/" title="blog or email this clip"><img src="http://content6.clipmarks.com/images/c2b-foot.png" border="0" alt="blog it" width="107" height="17" style="border-width:0px;padding:0px;margin:0px;" /></a></td></tr></table></div></div>

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