Amino Acid Derivatives Amino Acid Analysis HPLC and Separation of Derivatized Amino Acids


	Amino Acid Derivatives Amino Acid Analysis HPLC and Separation of Derivatized Amino Acids

biosynthesis

10-27-2009 5:06 AM

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amino acid derivatives

biosynthesis says:

Bio-Synthesis Inc. is a leading life science products company which deals in Amino Acid Derivatives, Amino Acid Analysis HPLC

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<div style="margin: 12px 0px; font-family: arial; color: #333333; background: #ffffff; border: solid 4px #e5e5e5; width: 100%; clear: left;"><div class="CM_CTB_Content_Wrap" style="margin: 0px; padding: 0px;background-color: #ffffff;"><div style="border-bottom: solid 1px #dcdcdc; white-space: nowrap; margin-bottom: 8px; background-color: #eeeeee ;background-image: url(http://clipmarks.com/images/source-bg.gif); background-repeat: repeat-x; height: 24px; line-height: 24px; vertical-align: middle; padding-bottom: 4px; color: #666666; font-size: 10px;" ><a href="http://clipmarks.com/clip-to-blog/" title="see clips that are hot right now"><img src="http://content.clipmarks.com/blog_embed/3ad4028c-63de-437a-878b-e616d09cccf7/2BECEE45-91EC-400C-A105-B4372D08CFF0/" alt="" width="19" height="19" border="0" style="vertical-align: middle; margin: 0px 4px; display: inline; border: none; float:none;" /></a>clipped from <a title="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx" href="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx" style="font-size: 11px;">www.biosyn.com</a></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><P>Amino  acids anlaysis at Biosynthesis are organic compounds made of carbon, hydrogen,  oxygen, nitrogen, and (in some cases) sulfur bonded in characteristic  formations. Strings of amino acids make up proteins, of which there are  countless varieties. Amino acid analysis is a  process to determine the quantities of each individual amino acid in a protein.</P></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><STRONG>Hydrolysis</STRONG></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><table background="undefined" bgcolor=""><tr><TD valign="top" align="left" class="bluefont">A known amount of internal standard (norleucine) is added to the sample.</TD></tr></table></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><STRONG>Derivatization</STRONG></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><table background="undefined" bgcolor=""><tr><TD valign="top" align="left" class="bluefont">PThe free amino acids cannot be detected by HPLC unless they have been  derivatized. Derivatization is performed automatically on the amino acid  analyzer by reacting the free amino acids, under basic conditions, with  phenylisothiocyanate (PITC) to produce phenylthiocarbamyl (PTC) amino acid  derivatives.  </TD></tr></table></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><STRONG>Separation of Derivatized Amino Acids</STRONG></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><table background="undefined" bgcolor=""><tr><TD valign="top" align="left" class="bluefont">The PTC-amino acids are separated on a reverse phase C18 silica column  and the PTC chromophore is detected at 254 nm. All of the amino acids will  elute in approximately 25 minutes.</TD></tr></table></blockquote><div style="height: 2px; font-size: 2px; background: #dcdcdc; border-bottom: solid 1px #f5f5f5; margin: 2px 4px;"></div><blockquote style="text-align: left; padding: 0px 8px; margin: 4px 0px 8px 0px; background: transparent; border: none;" cite="http://www.biosyn.com/landingpage/Amino_Acid_Analysis_Hplc.aspx"><div align="center"><img src="http://content9.clipmarks.com/blog_cache/www.biosyn.com/img/06BD7BCF-4260-42CA-8D3B-D445D73C2E88" alt="" /></div></blockquote></div><div style="margin: 0px 6px 6px 4px;"><table style="font-size: 11px;border-spacing: 0px;padding: 0px;" cellpadding="0" cellspacing="0" width="100%"><tr><td style="background:transparent;border-width:0px;padding:0px;">&nbsp;</td><td align="right" style="background:transparent;border-width:0px;padding:0px;width:107px" width="107"><a href="http://clipmarks.com/share/2BECEE45-91EC-400C-A105-B4372D08CFF0/blog/" title="blog or email this clip"><img src="http://content6.clipmarks.com/images/c2b-foot.png" border="0" alt="blog it" width="107" height="17" style="border-width:0px;padding:0px;margin:0px;" /></a></td></tr></table></div></div>

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